© 2003 Robert A. Freitas Jr. All Rights Reserved.

Robert A. Freitas Jr., Nanomedicine, Volume IIA: Biocompatibility, Landes Bioscience, Georgetown, TX, 2003

References 5700-5799

5700. M.E. McAlarney, M.A. Oshiro, L.P. Huang, “Effects of titanium oxide properties on biocompatibility. A preliminary study,” N.Y. State Dent. J. 59(November 1993):45-48.

5701. D.F. Williams, “Titanium: epitome of biocompatibility or cause for concern,” J. Bone Joint Surg. Br. 76(May 1994):348-349.

5702. M.T. Lawton, J.C. Ho, W.D. Bichard, S.W. Coons, J.M. Zabramski, R.F. Spetzler, “Titanium aneurysm clips: Part I – Mechanical, radiological, and biocompatibility testing,” Neurosurgery 38(June 1996):1158-1163, 1164 (discussion).

5703. X. Wang, H. Chen, G. Zhong, “The study of biocompatibility of titanium in middle ear cave of guinea-pig,” Lin Chuang Er Bi Yan Hou Ke Za Zhi 12(March 1998):134-136. In Chinese.

5704. K. Schwager, G. Geyer, “Titanium and glass-ionomer cement as ossicular replacement materials: biocompatibility results after implantation in the rabbit,” ORL J. Otorhinolaryngol. Relat. Spec. 60(November-December 1998):322-328.

5705. R.R. Wang, Y. Li, “In vitro evaluation of biocompatibility of experimental titanium alloys for dental restorations,” J. Prosthet. Dent. 80(October 1998):495-500.

5706. M. Assad, N. Lemieux, C.H. Rivard, L.H. Yahia, “Comparative in vitro biocompatibility of nickel-titanium, pure nickel, pure titanium, and stainless steel: genotoxicity and atomic absorption evaluation,” Biomed. Mater. Eng. 9(1999):1-12.

5707. A.D. Roddick-Lanzilotta, A.J. McQuillan, “An in situ infrared spectroscopic study of glutamic acid and of aspartic acid adsorbed on TiO(2): Implications for the biocompatibility of titanium,” J. Colloid Interface Sci. 227(1 July 2000):48-54.

5708. H. Matsuno, A. Yokoyama, F. Watari, M. Uo, T. Kawasaki, “Biocompatibility and osteogenesis of refractory metal implants, titanium, hafnium, niobium, tantalum and rhenium,” Biomaterials 22(June 2001):1253-1262.

5709. K. Derhami, J. Zheng, L. Li, J.F. Wolfaardt, P.G. Scott, “Proteomic analysis of human skin fibroblasts grown on titanium: novel approach to study molecular biocompatibility,” J. Biomed. Mater. Res. 56(August 2001):234-244.

5710. D. Krupa, J. Baszkiewicz, J.A. Kozubowski, A. Barcz, J.W. Sobczak, A. Bilinski, M. Lewandowska-Szumiel, B. Rajchel, “Effect of phosphorus-ion implantation on the corrosion resistance and biocompatibility of titanium,” Biomaterials 23(August 2002):3329-3340; “Effect of calcium-ion implantation on the corrosion resistance and biocompatibility of titanium,” Biomaterials 22(August 2001):2139-2151.

5711. Edward A. Evans, Urs Hafeli, Ram Wusirika, Philip W. Morrison, “Diamond like carbon coatings for rhenium wires and foils,” Proc. MRS Fall Meeting, Boston, MA, 1999; http://www.ecgf.uakron.edu/~chem/fclty/evans/u9.pdf

5712. “Diamond-like Carbon Coatings for Improved Wear Resistance of Textile Machine Parts BE S2 5049,” VITO - Vlaamse Instelling voor Technologisch Onderzoek NV, 1996; http://www.teresa-textile.net/projects/3_diwatex.htm

5713. Z.M. Zeng, X.B. Tian, T.K. Kwok, B.Y. Tang, M.K. Fung, P.K. Chu, “Effects of plasma excitation power, sample bias, and duty cycle on the structure and surface properties of amorphous carbon thin films fabricated on A1S1440 steel by plasma immersion ion implantation,” J. Vac. Sci. Technol. A 18(September 2000):2164-2168; http://www.cityu.edu.hk/ap/plasma/Publications/2000/00.11.pdf

5714. “Diamonex DLC Protective Coatings: Low-Temperature CVD Plasma and Ion-Beam Deposited Thin Film”; http://www.diamonex.com/protectivecoatings.html

5715. P. Bodin, G. Burnstock, “Evidence that release of adenosine triphosphate from endothelial cells during increased shear stress is vesicular,” J. Cardiovasc. Pharmacol. 38(December 2001):900-908.

5716. Jim Krane, “Manipulating molecules raises hopes, fears,” Associated Press, The Sacramento Bee, 9 September 2002, pp. D1, D4; http://seattletimes.nwsource.com/html/healthscience/134531866_bttoxins09.html; http://www.recordsearchlight.com/newsarchive/20020909topbus003.shtml; http://www.enn.com/news/wire-stories/2002/09/09062002/ap_48366.asp; http://www.enn.com/news/wire-stories/2002/09/09102002/ap_48381.asp. See also: Victoria Griffith, “Inside track: Big risks on a microscopic scale,” Financial Times (London), 25 September 2002.

5717. Mary Ellen Egan, “The plot thickens: Monitoring blood viscosity,” Forbes 170(16 September 2002):166; http://www.forbes.com/forbes/2002/0916/166_print.html; http://www.fibromyalgiasupport.com/library/showarticle.cfm/ID/3894/; see also http://www.rheologics.com

5718. M. Drukker, G. Katz, A. Urbach, M. Schuldiner, G. Markel, J. Itskovitz-Eldor, B. Reubinoff, O. Mandelboim, N. Benvenisty, “Characterization of the expression of MHC proteins in human embryonic stem cells,” Proc. Natl. Acad. Sci. (USA) 99(23 July 2002):9864-9869.

5719. Gretchen Vogel, “Stem cells not so stealthy after all,” Science 297(12 July 2002):175-177.

5720. J.C. Lee, H. Bermudez, B.M. Discher, M.A. Sheehan, Y.Y. Won, F.S. Bates, D.E. Discher, “Preparation, stability, and in vitro performance of vesicles made with diblock copolymers,” Biotechnol. Bioeng. 73(20 April 2001):135-145.

5721. D.E. Discher, A. Eisenberg, “Polymer vesicles,” Science 297(9 August 2002):967-973.

5722. P.R. Cullis, A. Chonn, S.C. Semple, “Interactions of liposomes and lipid-based carrier systems with blood proteins: Relation to clearance behaviour in vivo,” Adv. Drug Deliv. Rev. 32(8 June 1998):3-17.

5723. R. Lipowsky, E. Sackmann, eds., Structure and Dynamics of Membranes – from Cells to Vesicles, North-Holland, Amsterdam, 1995.

5724. M. Sferrazza, C. Xiao, R.A.L. Jones, D.G. Bucknall, J. Webster, J. Penfold, “Evidence for capillary waves at immiscible polymer/polymer interfaces,” Phys. Rev. Lett. 78(1997):3693-3696.

5725. A. Curtis, C. Wilkinson, “Topographical control of cells,” Biomaterials 18(December 1997):1573-1583.

5726. A. Curtis, C. Wilkinson, “New depths in cell behaviour: reactions of cells to nanotopography,” Biochem. Soc. Symp. 65(1999):15-26; A.S. Curtis, C.D. Wilkinson, “Reactions of cells to topography,” J. Biomater. Sci. Polym. Ed. 9(1998):1313-1329.

5727. R.G. Flemming, C.J. Murphy, G.A. Abrams, S.L. Goodman, P.F. Nealey, “Effects of synthetic micro- and nano-structured surfaces on cell behavior,” Biomaterials 20(March 1999):573-588.

5728. D. Williams, “Size and shape really matter: the influence of design on biocompatibility,” Med. Device Technol. 8(November 1997):8-12.

5729. Y. Ito, “Surface micropatterning to regulate cell functions,” Biomaterials 20(December 1999):2333-2342.

5730. B. Kasemo, J. Gold, “Implant surfaces and interface processes,” Adv. Dent. Res. 13(June 1999):8-20.

5731. A.S. Curtis, B. Casey, J.O. Gallagher, D. Pasqui, M.A. Wood, C.D. Wilkinson, “Substratum nanotopography and the adhesion of biological cells. Are symmetry or regularity of nanotopography important?” Biophys. Chem. 94(25 December 2001):275-283.

5732. M.J. Dalby, M.O. Riehle, H. Johnstone, S. Affrossman, A.S. Curtis, “In vitro reaction of endothelial cells to polymer demixed nanotopography,” Biomaterials 23(July 2002):2945-2954.

5733. M.J. Dalby, S.J. Yarwood, M.O. Riehle, H.J. Johnstone, S. Affrossman, A.S. Curtis, “Increasing fibroblast response to materials using nanotopography: morphological and genetic measurements of cell response to 13-nm-high polymer demixed islands,” Exp. Cell Res. 276(15 May 2002):1-9.

5734. B. Wojciak-Stothard, A. Curtis, W. Monaghan, K. MacDonald, C. Wilkinson, “Guidance and activation of murine macrophages by nanometric scale topography,” Exp. Cell Res. 223(15 March 1996):426-435.

5735. J.P. Ternaux, R. Wilson, J. Dow, A.S. Curtis, P. Clark, P. Portalier, J. Moores, “Dendritic processing: using microstructures to solve a hitherto intractable neurobiological problem,” Med. Biol. Eng. Comput. 30(July 1992):CE37-CE41.

5736. Y. Xie, T. Sproule, Y. Li, H. Powell, J.J. Lannutti, D.A. Kniss, “Nanoscale modifications of PET polymer surfaces via oxygen-plasma discharge yield minimal changes in attachment and growth of mammalian epithelial and mesenchymal cells in vitro,” J. Biomed. Mater. Res. 61(August 2002):234-245.

5737. C.S. Ranucci, P.V. Moghe, “Substrate microtopography can enhance cell adhesive and migratory responsiveness to matrix ligand density,” J. Biomed. Mater. Res. 54(February 2001):149-161.

5738. M.A. Wood, M. Riehle, C.D.W. Wilkinson, “Patterning colloidal nanotopographies,” Nanotechnology 13(2002):605-609.

5739. M.E. Akerman, W.C. Chan, P. Laakkonen, S.N. Bhatia, E. Ruoslahti, “Nanocrystal targeting in vivo,” Proc. Natl. Acad. Sci. (USA) 99(1 October 2002):12617-12621; http://www.pnas.org/cgi/content/full/99/20/12617

5740. X. Peng, L. Manna, W. Yang, J. Wickham, E. Scher, A. Kadavanich, A.P. Alivisatos, “Shape control of CdSe nanocrystals,” Nature 404(2 March 2000):59-61.

5741. J.R. Taylor, M.M. Fang, S. Nie, “Probing specific sequences on single DNA molecules with bioconjugated fluorescent nanoparticles,” Anal. Chem. 72(1 May 2000):1979-1986.

5742. M. Qhobosheane, S. Santra, P. Zhang, W. Tan, “Biochemically functionalized silica nanoparticles,” Analyst 126(August 2001):1274-1278.

5743. E.R. Goldman, G.P. Anderson, P.T. Tran, H. Mattoussi, P.T. Charles, J.M. Mauro, “Conjugation of luminescent quantum dots with antibodies using an engineered adaptor protein to provide new reagents for fluoroimmunoassays,” Anal. Chem. 74(15 February 2002):841-847.

5744. D. Gerion, W.J. Parak, S.C. Williams, D. Zanchet, C.M. Micheel, A.P. Alivisatos, “Sorting fluorescent nanocrystals with DNA,” J. Am. Chem. Soc. 124(19 June 2002):7070-7074.

5745. L.Y. Wang, L. Wang, F. Gao, Z.Y. Yu, Z.M. Wu, “Application of functionalized CdS nanoparticles as fluorescence probe in the determination of nucleic acids,” Analyst 127(July 2002):977-980.

5746. S.R. Sershen, S.L. Westcott, N.J. Halas, J.L. West, “Temperature-sensitive polymer-nanoshell composites for photothermally modulated drug delivery,” J. Biomed. Mater. Res. 51(5 September 2000):293-298.

5747. L.C. Bowles, R.A. Bell, M.J. Ernste, J.R. Kramer, H. Manolopoulos, N. Ogden, “Synthesis and characterization of metal sulfide clusters for toxicological studies,” Environ. Toxicol. Chem. 21(April 2002):693-699.

5748. C.L. Torres-Martinez, R. Kho, O.I. Mian, R.K. Mehra, “Efficient photocatalytic degradation of environmental pollutants with mass-produced ZnS nanocrystals,” J. Colloid Interface Sci. 240(15 August 2001):525-532.

5749. J. Aldana, Y.A. Wang, X. Peng, “Photochemical instability of CdSe nanocrystals coated by hydrophilic thiols,” J. Am. Chem. Soc. 123(12 September 2001):8844-8850.

5750. Y. Zhang, N. Kohler, M. Zhang, “Surface modification of superparamagnetic magnetite nanoparticles and their intracellular uptake,” Biomaterials 23(April 2002):1553-1561.

5751. E. Ruoslahti, D. Rajotte, “An address system in the vasculature of normal tissues and tumors,” Annu. Rev. Immunol. 18(2000):813-827.

5752. E. Ruoslahti, “Targeting tumor vasculature with homing peptides from phage display,” Semin. Cancer Biol. 10(December 2000):435-442.

5753. W. Arap, W. Haedicke, M. Bernasconi, R. Kain, D. Rajotte, S. Krajewski, H.M. Ellerby, D.E. Bredesen, R. Pasqualini, E. Ruoslahti, “Targeting the prostate for destruction through a vascular address,” Proc. Natl. Acad. Sci. (USA) 99(5 February 2002):1527-1531; http://www.pnas.org/cgi/content/full/99/3/1527

5754. M. Essler, E. Ruoslahti, “Molecular specialization of breast vasculature: a breast-homing phage-displayed peptide binds to aminopeptidase P in breast vasculature,” Proc. Natl. Acad. Sci. (USA) 99(19 February 2002):2252-2257; http://www.pnas.org/cgi/content/full/99/4/2252

5755. K. Porkka, P. Laakkonen, J.A. Hoffman, M. Bernasconi, E. Ruoslahti, “A fragment of the HMGN2 protein homes to the nuclei of tumor cells and tumor endothelial cells in vivo,” Proc. Natl. Acad. Sci. (USA) 99(28 May 2002):7444-7449; http://www.pnas.org/cgi/content/full/99/11/7444

5756. P. Laakkonen, K. Porkka, J.A. Hoffman, E. Ruoslahti, “A tumor-homing peptide with a targeting specificity related to lymphatic vessels,” Nat. Med. 8(July 2002):751-755.

5757. “Homing peptides mounted on semiconductor particles deliver tiny payload to cancerous tissue in live mice,” AScribe Newswire, 11 September 2002; http://www.technologyreview.com/offthewire/3001_1192002_2.asp

5758. L.M. Kasman, A. Kasman, C. Westwater, J. Dolan, M.G. Schmidt, J.S. Norris, “Overcoming the phage replication threshold: a mathematical model with implications for phage therapy,” J. Virol. 76(June 2002):5557-5564.

5759. A. Pirisi, “Phage therapy – advantages over antibiotics?” Lancet 356(21 October 2000):1418.

5760. B. Weber-Dabrowska, M. Zimecki, M. Mulczyk, A. Gorski, “Effect of phage therapy on the turnover and function of peripheral neutrophils,” FEMS Immunol. Med. Microbiol. 34(11 October 2002):135-138.

5761. L. Broxmeyer, D. Sosnowska, E. Miltner, O. Chacon, D. Wagner, J. McGarvey, R.G. Barletta, L.E. Bermudez, “Killing of Mycobacterium avium and Mycobacterium tuberculosis by a mycobacteriophage delivered by a nonvirulent mycobacterium: A model for phage therapy of intracellular bacterial pathogens,” J. Infect. Dis. 186(15 October 2002):1155-1160.

5762. R. Stone, “Bacteriophage therapy. Food and agriculture: testing grounds for phage therapy,” Science 298(25 October 2002):730.

5763. K.E. Cerveny, A. DePaola, D.H. Duckworth, P.A. Gulig, “Phage therapy of local and systemic disease caused by Vibrio vulnificus in iron-dextran-treated mice,” Infect. Immun. 70(November 2002):6251-6262.

5764. P.J. Newman, “The biology of PECAM-1,” J. Clin. Invest. 99(1 January 1997):3-8; http://www.jci.org/cgi/content/full/99/1/3

5765. J.F. Kerr, A.H. Wyllie, A.R. Currie, “Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics,” Br. J. Cancer 26(August 1972):239-257.

5766. J. Savill, V. Fadok, “Corpse clearance defines the meaning of cell death,” Nature 407(12 October 2000):784-788.

5767. D.R. Green, H.M. Beere, “Apoptosis. Mostly dead,” Nature 412(12 July 2001):133-135.

5768. K. Fujiwara, M. Masuda, M. Osawa, Y. Kano, K. Katoh, “Is PECAM-1 a mechanoresponsive molecule?” Cell Struct. Funct. 26(February 2001):11-17.

5769. Y. Ren, J. Savill, “Apoptosis: the importance of being eaten,” Cell Death Differ. 5(July 1998):563-568.

5770. H.W. Fink, C. Schonenberger, “Electrical conduction through DNA molecules,” Nature 398(1 April 1999):407-410.

5771. D. Porath, A. Bezryadin, S. de Vries, C. Dekker, “Direct measurement of electrical transport through DNA molecules,” Nature 403(10 February 2000):635-638.

5772. D.T. Odom, E.A. Dill, J.K. Barton, “Robust charge transport in DNA double crossover assemblies,” Chem. Biol. 7(July 2000):475-481.

5773. K.H. Yoo, D.H. Ha, J.O. Lee, J.W. Park, J. Kim, J.J. Kim, H.Y. Lee, T. Kawai, H.Y. Choi, “Electrical conduction through poly(dA)-poly(dT) and poly(dG)-poly(dC) DNA molecules,” Phys. Rev. Lett. 87(5 November 2001):198102; http://phys.chonbuk.ac.kr/quantum/pdf/PRLDNA.pdf

5774. D.T. Odom, E.A. Dill, J.K. Barton, “Charge transport through DNA four-way junctions,” Nucleic Acids Res. 29(15 May 2001):2026-2033; http://nar.oupjournals.org/cgi/content/full/29/10/2026

5775. Mattias Hjort, Sven Stafstrom, “Band resonant tunneling in DNA molecules,” Phys. Rev. Lett. 87(26 November 2001):228101; http://www.ifm.liu.se/~mathj/papers/art5.pdf

5776. E.M. Boon, J.K. Barton, “Charge transport in DNA,” Curr. Opin. Struct. Biol. 12(June 2002):320-329.

5777. Michael L. Weiner, Patrick R. Connelly, Carol Gebert, Robert W. Gray, “Hybrid Nucleic Acid Assembly,” International Patent Publication Number WO 01/60969 A1, 23 August 2001, Application Number PCT/US01/05139; http://www.biomedsolutions.com

5778. T. Tudoriu, C. Ionescu, H. Angelescu, “Protheses of the penis; 130 cases,” Zentralbl. Chir. 103(1978):901-910. In German.

5779. K. Ganabathi, R. Dmochowski, P.E. Zimmern, G.E. Leach, “Peyronie’s disease: surgical treatment based on penile rigidity,” J. Urol. 153(March 1995):662-666.

5780. J. Wironen, J. Marotta, M. Cohen, C. Batich, “Materials used in urological devices,” J. Long Term Eff. Med. Implants 7(1997):1-28.

5781. L.A. Levine, R.J. Dimitriou, “A surgical algorithm for penile prosthesis placement in men with erectile failure and Peyronie’s disease,” Int. J. Impot. Res. 12(June 2000):147-151.

5782. L. Werner, J.M. Legeais, M.D. Nagel, G. Renard, “Neutral red assay of the cytotoxicity of fluorocarbon-coated polymethylmethacrylate intraocular lenses in vitro,” J. Biomed. Mater. Res. 48(1999):814-819.

5783. M.W. Hall, K.A. Solen, “Hypothermia-induced platelet aggregation in human blood in an in vitro model: the dominant role of blood-material interactions,” J. Biomed. Mater. Res. 59(5 March 2002):528-534.

5784. S.B. Khoo, F. Chen, “Studies of sol-gel ceramic film incorporating methylene blue on glassy carbon: an electrocatalytic system for the simultaneous determination of ascorbic and uric acids,” Anal. Chem. 74(15 November 2002):5734-5741.

5785. B.I. Silveira, A.E. Dantas, J.E. Blasquez, R.K. Santos, “Characterization of inorganic fraction of spent potliners: evaluation of the cyanides and fluorides content,” J. Hazard Mater. 89(28 January 2002):177-183.

5786. D.G. Maki, M. Ringer, “Risk factors for infusion-related phlebitis with small peripheral venous catheters. A randomized controlled trial,” Ann. Intern. Med. 114(15 May 1991):845-854.

5787. M. Talja, M. Ruutu, L.C. Andersson, O. Alfthan, “Urinary catheter structure and testing methods in relation to tissue toxicity,” Br. J. Urol. 58(August 1986):443-449.

5788. C.J. Doillon, K. Cameron, “New approaches for biocompatibility testing using cell culture,” Int. J. Artif. Organs 13(August 1990):517-520; M.A. Rathbun, R.G. Craig, C.T. Hanks, F.E. Filisko, “Cytotoxicity of a BIS-GMA dental composite before and after leaching in organic solvents,” J. Biomed. Mater. Res. 25(April 1991):443-457.

5789. M.V. Risbud, S. Hambir, J. Jog, R. Bhonde, “Biocompatibility assessment of polytetrafluoroethylene/wollastonite composites using endothelial cells and macrophages,” J. Biomater. Sci. Polym. Ed. 12(2001):1177-1189.

5790. Steven S. Smith, The Expression of beta-galactosidase during Continuous Culture on Mixed-Substrate Limited Media, Ph.D. Thesis in Molecular Biology, University of California at Los Angeles, 1974, pp. 70-73.

5791. S.S. Smith, D.E. Atkinson, “The expression of beta-galactosidase by Escherichia coli during continuous culture,” Arch. Biochem. Biophys. 202(July 1980):573-581.

5792. J. Davenas, P. Thevenard, F. Philippe, M.N. Arnaud, “Surface implantation treatments to prevent infection complications in short term devices,” Biomol. Eng. 19(August 2002):263-268.

5793. S.H. Park, A.E. Aust, “Participation of iron and nitric oxide in the mutagenicity of asbestos in hgprt-, gpt+ Chinese hamster V79 cells,” Cancer Res. 58(15 March 1998):1144-1148.

5794. Z. Shen, D. Bosbach, M.F. Hochella Jr., D.L. Bish, M.G. Williams Jr., R.F. Dodson, A.E. Aust, “Using in vitro iron deposition on asbestos to model asbestos bodies formed in human lung,” Chem. Res. Toxicol. 13(September 2000):913-921.

5795. M.J. O’Connell, S.M. Bachilo, C.B. Huffman, V.C. Moore, M.S. Strano, E.H. Haroz, K.L. Rialon, P.J. Boul, W.H. Noon, C. Kittrell, J. Ma, R.H. Hauge, R.B. Weisman, R.E. Smalley, “Band gap fluorescence from individual single-walled carbon nanotubes,” Science 297(26 July 2002):593-596.

5796. H. Suzuki, M. Takei, T. Nakahata, H. Fukamachi, “Inhibitory effect of adenosine on degranulation of human cultured mast cells upon cross-linking of Fc epsilon RI,” Biochem. Biophys. Res. Commun. 242(26 January 1998):697-702.

5797. H.H. Jacobi, P.S. Skov, L.K. Poulsen, H.J. Malling, N. Mygind, “Histamine and tryptase in nasal lavage fluid after allergen challenge: effect of 1 week of pretreatment with intranasal azelastine or systemic cetirizine,” J. Allergy Clin. Immunol. 103(May 1999):768-772.

5798. H. Suzuki, A. Ueno, M. Takei, K. Shindo, T. Higa, H. Fukamachi, “The effects of S1319, a novel marine sponge-derived beta2-adrenoceptor agonist, on IgE-mediated activation of human cultured mast cells,” Inflamm. Res. 49(February 2000):86-94.

5799. T. Zuberbier, S.U. Chong, K. Grunow, S. Guhl, P. Welker, M. Grassberger, B.M. Henz, “The ascomycin macrolactam pimecrolimus (Elidel, SDZ ASM 981) is a potent inhibitor of mediator release from human dermal mast cells and peripheral blood basophils,” J. Allergy Clin. Immunol. 108(August 2001):275-280.

Last updated on 16 April 2004